27th – 28th September 2021
Module I – Theory
Fundamentals of High-Resolution Mass Spectrometry-based Proteomics
Sample preparation for Mass Spectrometry-based Proteomics
Ultrasonic-assisted digestions – Proteosonics
Protein identification by Mass Spectrometry
nanoESI-QTOF and Label Free Quantification
Module II – Hand-on
Sample preparation: high throughput in-solution digestion
Data acquisition and analysis: Protein identification and quantification
Data analysis: Perseus and Cytoscape
The course is 40% hands-on in the laboratory (live video streaming, participants work in their own laboratory) | 40% bioinformatic tools and 20% theory.
Participants needs to have access to one laboratory. All samples and necessary reagents are delivered by us.
The course is based on the following recently published work:
Ultrasonic-Based Filter Aided Sample Preparation as the General Method to Sample Preparation in Proteomic.
Anal. Chem. 2020, 92, 13, 9164–9171, https://doi.org/10.1021/acs.analchem.0c01470